Experiments involving the reduction of the blue dye DPIP, which loses its blue color and becomes clear as it absorbs electrons, have shown that the dye shows the greatest loss of color when in the presence of chloroplasts
- under bright, white light
- in a dark box
- that have been boiled before exposure to light
- exposed only to green light
- in a sugar solution
Answer(s): A
Explanation:
If DPIP is reduced (gains electrons) to lose its color, then the DPIP will show the greatest loss of color wherever the greatest number of electrons are being knocked around. Chlorophyll loses electrons when it is bombarded by photons of light. Thus, only chlo-roplasts in bright, white light will change DPIP greatly. Boiled chloroplasts will be ineffective due to enzyme denaturation, and chloroplasts in the dark or exposed only to green light will absorb few photons if any. Chloroplasts have no direct effect on sugar production; thus being in a sugar solution should not affect this reaction with DPIP in any way.