QUESTION: 45 Exam Topic: 1, Biology

In order to splice a foreign gene into a bacterial plasmid in the laboratory to create a recombinant plasmid, one would use

reverse transcriptase
DNA polymerase
catalase
polymerase chain reaction (PCR)
restriction enzymes

Answer(s): E

Explanation:

Restriction enzymes are specific for cutting certain sequences of DNA and are used for making recombinant DNA. Reverse transcriptase is a viral enzyme used to convert RNA DNA; DNA polymerase is used to copy and replicate DNA; catalase is used in cells to break down hydrogen peroxide, a dangerous side-product of many cellular reactions; and, PCR is used to amplify (copy) short segments of DNA.

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QUESTION: 46 Exam Topic: 1, Biology

The process by which large fats are broken down into smaller "droplets" of fat for easier chemical digestion within the small intestine is called

lipation
emulsification
hydrolysis
dehydration synthesis
cleavage

Answer(s): B

Explanation:

Emulsification is the term used for breaking down fats into smaller pieces to facilitate chemical digestion. Bile, secreted by the liver, is responsible for doing this within the lumen of the small intestine.

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